WGA試劑盒
主模板直接擴增法(Primary Template-directed Amplification, PTA)是一個全新的等溫全基因組擴增WGA方法，能夠覆蓋到單細胞基因組>95%的區域，重現性、均一性和準確性均優于傳統擴增法，具體體現在變異位點檢測的靈敏度和特異性上，降低了測序數據量，方便后續生信數據分析。PTA以其技術上卓越的性能而適用不同的應用場景：
1)單細胞和低起始量樣品中準確檢測變異（SNPs, indel, SNVs 和CNVs）；
2)檢測和鑒定最小病灶殘留MRD；
3)全基因組范圍內定量評估CRISPR/Cas9基因編輯效果，分辨率可以到每個單細胞；
4)基因組組裝和鑒定罕見的不能培養的微生物；
參考文獻：https://www.biorxiv.org/content/10.1101/2020.11.20.391961v1
訂購指南：order@4basedx.com
貨號 | 品名描述 |
100068/100136 | ResolveDNA Whole Genome Amplification Kit – For single-cell and low-input DNA amplification (24 or 96 rxns) |
100121/100182 | ResolveDNA Bead Purification Kit – For reliable WGA reaction and library cleanup (24 or 96 rxns) |
100135 | ResolveDNA Magnetic Plate – For optimum bead cleanup performance |
100226 | ResolveDNA Dual Volume Strip Tube Magnet – For optimum bead cleanup performance |
100137 | ResolveDNA Complete Starter Pack – Cell sorting and WGA equipment, reagents and consumables |
100180 | ResolveDNA? Consumables Only Starter Pack – Cell sorting and WGA consumables |
100181 | ResolveDNA? 10x Library Adapters – Compatible with Illumina sequencers |
100199 | ResolveDNA? FACS Kit – For optimum FACS-based cell sorting |
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